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NAD Legit A transparency-first ledger of the NAD+ evidence — what the controlled human trials actually show, what is sold as a supplement versus approved as a drug, and where the IV-quality and 'unproven-benefit' risks really sit.

07. Studies behind the answers

NAD+ references say whether studies used people or animals

Each paper has a direct link. You can tell whether the paper tested cells in a dish, mice, or people.

Go back to the human resultsRead the plain answers

How to use the study list

The number beside a claim matches an entry below. The matching entry gives the paper's title, a link, and what the researchers tested.

Some studies used people, while others used mice and cells studied outside a body, which can't tell you how your own health will change later after a pill or drip. The kind of study matters.

Some paper titles include a tested brand. We keep the published title, but we don't back the brand.

  1. Yoshino M, Yoshino J, Kayser BD, et al. Nicotinamide mononucleotide increases muscle insulin sensitivity in prediabetic women. Science. 2021;372(6547):1224-1229. ↗
  2. Camacho-Pereira J, Tarragó MG, Chini CCS, et al. CD38 Dictates Age-Related NAD Decline and Mitochondrial Dysfunction through an SIRT3-Dependent Mechanism. Cell Metab. 2016;23(6):1127-1139. ↗
  3. Yi L, Maier AB, Tao R, et al. The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial. GeroScience. 2023;45(1):29-43. ↗
  4. Conze D, Brenner C, Kruger CL. Safety and Metabolism of Long-term Administration of NIAGEN (Nicotinamide Riboside Chloride) in a Randomized, Double-Blind, Placebo-controlled Clinical Trial of Healthy Overweight Adults. Sci Rep. 2019;9(1):9772. ↗
  5. Covarrubias AJ, Perrone R, Grozio A, Verdin E. NAD+ metabolism and its roles in cellular processes during ageing. Nat Rev Mol Cell Biol. 2021;22(2):119-141. ↗
  6. Martens CR, Denman BA, Mazzo MR, et al. Chronic nicotinamide riboside supplementation is well-tolerated and elevates NAD+ in healthy middle-aged and older adults. Nat Commun. 2018;9(1):1286. ↗
  7. Irie J, Inagaki E, Fujita M, et al. Effect of oral administration of nicotinamide mononucleotide on clinical parameters and nicotinamide metabolite levels in healthy Japanese men. Endocr J. 2020;67(2):153-160. ↗
  8. Grant R, Berg J, Mestayer R, et al. A Pilot Study Investigating Changes in the Human Plasma and Urine NAD+ Metabolome During a 6 Hour Intravenous Infusion of NAD+. Front Aging Neurosci. 2019;11:257. ↗
  9. Braidy N, Villalva MD, van Eeden S. Sobriety and Satiety: Is NAD+ the Answer? Antioxidants (Basel). 2020;9(5):425. ↗
  10. Cameron AM, Castoldi A, Sanin DE, et al. Inflammatory macrophage dependence on NAD+ salvage is a consequence of reactive oxygen species-mediated DNA damage. Nat Immunol. 2019;20(4):420-432. ↗
  11. Fouquerel E, Sobol RW. ARTD1 (PARP1) activation and NAD+ in DNA repair and cell death. DNA Repair (Amst). 2014;23:27-32. ↗
  12. Herrmann GK, Russell WK, Garg NJ, Yin YW. Poly(ADP-ribose) polymerase 1 regulates mitochondrial DNA repair in an NAD-dependent manner. J Biol Chem. 2021;296:100309. ↗
  13. Navarro MN, Gómez de Las Heras MM, Mittelbrunn M. Nicotinamide adenine dinucleotide metabolism in the immune response, autoimmunity and inflammageing. Br J Pharmacol. 2022;179(9):1839-1856. ↗
  14. Vinten KT, Trętowicz MM, Coskun E, et al. NAD+ precursor supplementation in human ageing: clinical evidence and challenges. Nat Metab. 2025;7(10):1974-1990. ↗
  15. Reyna K, Mestayer R, Mestayer P, et al. Intravenous infusion of nicotinamide adenine dinucleotide (NAD+) versus nicotinamide riboside (NR): a retrospective tolerability pilot study in a real-world setting. Front Aging. 2026;7:1652582. ↗